Aug 17, 2020

Public workspaceBasic Molecular Biology V.2

DOI
dx.doi.org/10.17504/protocols.io.bc64izgw
  • 1Brigham Young University
Ken Christensen
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DOI: dx.doi.org/10.17504/protocols.io.bc64izgw
Collection Citation: Ken Christensen 2020. Basic Molecular Biology. protocols.io https://dx.doi.org/10.17504/protocols.io.bc64izgw
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 03, 2020
Last Modified: August 17, 2020
Collection Integer ID: 33724
Abstract
A collection of public protocols used in the Christensen Lab.
Files
Protocol
Icon representing the file Bacterial transformation
Name
Bacterial transformation
Version 2
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Agarose Gel Electrophoresis
Name
Agarose Gel Electrophoresis
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Electroporation Protocol
Name
Electroporation Protocol
Version 1
, New England Biolabs
New England BiolabsNew England Biolabs
Protocol
Icon representing the file Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids
Name
Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Diagnostic Restriction Digest
Name
Diagnostic Restriction Digest
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Restriction Digest
Name
Restriction Digest
Version 2
, New England Biolabs
New England BiolabsNew England Biolabs
Protocol
Icon representing the file DNA Ligation
Name
DNA Ligation
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file DNA Quantification
Name
DNA Quantification
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file How to Design a Primer
Name
How to Design a Primer
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Primer Design for Restriction Enzyme Cloning (E6901)
Name
Primer Design for Restriction Enzyme Cloning (E6901)
Version 1
, New England Biolabs
New England BiolabsNew England Biolabs
Protocol
Icon representing the file Inoculating a Liquid Bacterial Culture
Name
Inoculating a Liquid Bacterial Culture
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Pouring LB Agar Plates
Name
Pouring LB Agar Plates
Version 3
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Polymerase Chain Reaction (PCR)
Name
Polymerase Chain Reaction (PCR)
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Purifying DNA from an Agarose Gel
Name
Purifying DNA from an Agarose Gel
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Sequence Analysis of a Plasmid
Name
Sequence Analysis of a Plasmid
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Streaking and Isolating Bacteria on an LB Agar Plate
Name
Streaking and Isolating Bacteria on an LB Agar Plate
Version 1
, Addgene
Addgene The Nonprofit Plasmid RepositoryAddgene
Protocol
Icon representing the file Making your own electrocompetent cells
Name
Making your own electrocompetent cells
Version crtv6m
, New England Biolabs
New England BiolabsNew England Biolabs
Protocol
Icon representing the file Drop Dialysis
Name
Drop Dialysis
Version 1
, Brigham Young University
Ken ChristensenBrigham Young University