This protocol is for performing Polymerase Chain Reaction (PCR). To see the full abstract and additional resources, visit https://www.addgene.org/protocols/pcr/.Basic PCR ProgramInitial Denaturation for at : This initiation step heats the double stranded DNA template strand to the point where the strands start denaturing and the hydrogen bonds are broken between the nucleotide base pairs.Denature at : Continued denaturation of double stranded DNA.Anneal primers for at : The forward and reverse primers are stable within this temperature range to anneal to each of the single stranded DNA template strands. The DNA polymerase is also stable enough to now bind to the primer DNA sequence.Extend DNA for at : The Taq polymerase has an optimal temperature around - so this step enables the DNA polymerase to synthesize and elongate the new target DNA strand accurately and rapidly.Repeat steps 2-5, 25-30 times.Final Extension for at : A final extension to fill-in any protruding ends of the newly synthesized strands.