Mar 20, 2023

YT medium

DOI
https://dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
YT medium
  • 1University of Oslo
Andreas Sagen
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DOI: https://dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
Protocol Citation: Andreas Sagen 2023. YT medium. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygx9qx4g8j/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 06, 2023
Last Modified: March 20, 2023
Protocol Integer ID: 78208
Keywords: YT medium, YT, e. coli, Escherichia coli, medium, microbial growth, yt medium, rich liquid microbial growth medium, escherichia coli, propagation of recombinant strain, m13 bacteriophage, strain, recombinant strain, dna bacteriophage, stranded dna bacteriophage
Abstract
2xYT is a nutritionally rich liquid microbial growth medium used for propagation of recombinant strains of Escherichia coli and M13 bacteriophage or other filamentous single-stranded DNA bacteriophages.
Guidelines
Prepare enough for the necessary number of experiments to perform in order to prevent batch-to-batch variation between experiments. Input chemicals should be of a certain quality in order to prevent contaminants that can impact microbial growth and selection.
Materials
Magnetic stirrer, autoclave, scale, tubes, flask
Protocol materials
Yeast ExtractMerck MilliporeSigma (Sigma-Aldrich)Catalog #Y0875
Sodium chlorideMerck MilliporeSigma (Sigma-Aldrich)Catalog #S9625
TryptoneMerck Millipore (EMD Millipore)Catalog #T9410
Safety warnings
Be sure to wear appropriate PPE when working with antibiotics and chemicals. Take care when working with hot flasks and tubes.
500 mL 2x YT medium
15m
Fill the bottle with 400 mL deionized water
5m
Measure and add:
8 g Tryptone
5 g Yeast extract
2.5 g Sodium chloride

Powdered compounds:
TryptoneMerck Millipore (EMD Millipore)Catalog #T9410
Yeast ExtractMerck MilliporeSigma (Sigma-Aldrich)Catalog #Y0875
Sodium chlorideMerck MilliporeSigma (Sigma-Aldrich)Catalog #S9625
15m
Adjust pH to 7.0 by adding drops of concentrated NaOH
5m
Add deionized water to a total of 500 mL
5m
Autoclave liquid at 121 °C for 00:15:00

Note
Cool to 50 °C and supplement with antibiotics as appropriate


15m