Preparation of Yeast-Sucrose-Broth, that is used as liquid media for growing Zt. This is called 'YSB' in Bruce McDonald lab. Have a look for example: Zain, M. E., et al. 'Influence of growth medium on diagnostic characters of Aspergillus and Penicillium species.' African Journal of Microbiology Research 3.5 (2009): 280-286.