Jun 28, 2024
Yale Murine TMC - H&E staining following Phenocycler-Fusion Imaging
- Jungmin Nam1,
- Yale Pathology Tissue Services1,
- Rong Fan1,
- Archibald Enninful1
- 1Yale University
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: Jungmin Nam, Yale Pathology Tissue Services, Rong Fan, Archibald Enninful 2024. Yale Murine TMC - H&E staining following Phenocycler-Fusion Imaging. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov19zjklr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 27, 2024
Last Modified: June 28, 2024
Protocol Integer ID: 102545
Keywords: phenocycler-fusion, codex, h&e, fusion imaging flow cell removal after phenocycler, fusion imaging flow cell removal, steps from yale pathology tissue service, yale pathology tissue service, fusion imaging, yale murine tmc, following phenocycler, pathology, staining step, phenocycler
Funders Acknowledgements:
Vishwa Deep Dixit
Grant ID: U54 AG079759
Abstract
Flow cell removal after Phenocycler-Fusion imaging and following H&E staining steps from Yale Pathology Tissue Services (YPTS).
Troubleshooting
Removing flow cell following CODEX imaging
Remove slides from storage buffer.
Immerse slides to xylene for 10 mins.
10m
Separate flow cell from the slide using a razorblades.
Rinse slide with 1x PBS.
Store in storage buffer until H&E staining.
Hematoxylin and eosin staining
11m 30s
Stain with hematoxylin for 5 min.
5m
Rinse with water.
Clarifier for 30 seconds.
30s
Rinse with water.
Bluing for 1 min.
1m
Rinse with water.
Stain with eosin 5 min.
5m
Immerse slides in graded ethanol to xylene.
Add coverslip with resin mounting medium.