Jun 13, 2022

Public workspaceWorm Freezing Buffer

DOI
dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
  • 1Baylor College of Medicine
  • Adrien Assie: Uploader to Protocols.io, original protocol found on wormbook from Brener et al. 1974
  • Buck Samuel: Uploader to Protocols.io, original protocol found on wormbook from Brener et al. 1974
Adrien Assie
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DOI: dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
External link: http://www.wormbook.org/chapters/www_strainmaintain/strainmaintain.html#d0e920
Protocol Citation: Adrien Assie, Buck Samuel 2022. Worm Freezing Buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.14egnxjrzl5d/v1
Manuscript citation:
Brenner, S. (1974). Genetics 77, 71
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 11, 2019
Last Modified: June 13, 2022
Protocol Integer ID: 22275
Disclaimer
Original protocol found on wormbook from Brener et al. 1974

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Abstract
Worm Freezing buffer recipe according to Brenner, S. (1974). Genetics77, 71. Found on wormbook see external link
Amount150 mL of Concentration100 % volume glycerol


Amount25 mL of Concentration1 Molarity (M) Potassium Phosphate Buffer, pH 6.0


Amount2.85 g of NaCl

Amount500 mL of M9 Buffer




Adjust deionized water toAmount1000 mL

Autoclave