Mar 21, 2025
Whole Genome Sequencing preparation
This protocol is a draft, published without a DOI.
- Fabian Grubert1
- 1Stanford
Protocol Citation: Fabian Grubert 2025. Whole Genome Sequencing preparation. protocols.io https://protocols.io/view/whole-genome-sequencing-preparation-d53y88pw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 21, 2025
Last Modified: March 21, 2025
Protocol Integer ID: 124760
Keywords: whole genome sequencing preparation genomic dna, tissue dna isolation micro kit, extracted dna, norgen biotek cell, cells per specimen, sequencing, dna, kapa hyperprep kit, illumina novaseq, cycles kit, genome
Abstract
Genomic DNA was extracted from 1 million cells per specimen, using the Norgen Biotek Cells and Tissue DNA Isolation Micro Kit. The extracted DNA was then fragmented using a Covaris ultrasonicator and subjected to library prep following the KAPA HyperPrep Kit. The final libraries were sequenced on Illumina NovaSeq with either S2 or S4 flowcells with the 300 cycles kit.
Troubleshooting
Abstract
Genomic DNA was extracted from 1 million cells per specimen, using the Norgen Biotek Cells and Tissue DNA Isolation Micro Kit. The extracted DNA was then fragmented using a Covaris ultrasonicator and subjected to library prep following the KAPA HyperPrep Kit. The final libraries were sequenced on Illumina NovaSeq with either S2 or S4 flowcells with the 300 cycles kit.