Composition of alcohol-based formulations for in-house/local production
The choice of components for WHO handrubs takes into account both cost constraints and microbiological efficacy. The procurement of raw ingredients will be influenced by the availability of sub-standard materials on the market and it is important to select local sources with care.
The following two alcohol-based handrub formulations are recommended for preparation in-house or in a local production facility, up to a maximum of 50 litres:
To produce final concentrations of ethanol 80% v/v, glycerol 1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v.
To produce final concentrations of isopropyl alcohol 75% v/v, glycerol 1.45% v/v, hydrogen peroxide (H2O2) 0.125% v/v: Only pharmacopoeial quality reagents should be used (e.g. The International Pharmacopoeia) and not technical grade products
WHO 2020. WHO-recommended handrub formulations. protocols.iohttps://dx.doi.org/10.17504/protocols.io.bdqei5te
This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
CreatedMar 15, 2020
Last ModifiedMar 15, 2020
PROTOCOL integer ID34278
|REAGENTS FOR FORMULATION 1:||REAGENTS FOR FORMULATION 2:|
|• Ethanol 96%||• Isopropyl alcohol 99.8%|
|• Hydrogen peroxide 3%||• Hydrogen peroxide 3%|
|• Glycerol 98%||• Glycerol 98%|
|• Sterile distilled or boiled cold water||• Sterile distilled or boiled cold water|
- 10-litre glass or plastic bottles with screw-threaded stoppers, or
- 50-litre plastic tanks (preferably in polypropylene or high density polyethylene, translucent so as to see the liquid level), or
- Stainless steel tanks with a capacity of 80–100 litres (for mixing without overflowing)
- Wooden, plastic or metal paddles for mixing
- Measuring cylinders and measuring jugs
- Plastic or metal funnel
- 100 ml plastic bottles with leak-proof tops
- 500 ml glass or plastic bottles with screw tops
- An alcoholometer: the temperature scale is at the bottom and the ethanol concentration (percentage v/v) at the top
Method for 10 L preparations
Step 1 includes a Step case.
Alcohol: Ethanol 96%
Pour 8333 mL Ethanol 96% into the large bottle or tank up to the graduated mark.
Add 417 mL Hydrogen peroxide 3% using the measuring cylinder.
Add 145 mL Glycerol using a measuring cylinder.
Top the bottle/tank up to the 10-liter mark with sterile distilled or cold boiled water.
Place the lid or the screw cap on the tank/bottle as soon as possible after preparation, in order to prevent evaporation.
Mix the solution by shaking gently where appropriate or by using a paddle.
Immediately divide up the solution into its final containers.
Place the bottles in quarantine for 72:00:00 before use.