Jan 17, 2018
WB SDS loading buffer (5X)
- 1University of California, San Francisco
- Stephen Floor Lab
Protocol Citation: Bao Thai 2018. WB SDS loading buffer (5X). protocols.io https://dx.doi.org/10.17504/protocols.io.mn9c5h6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 17, 2018
Last Modified: March 28, 2018
Protocol Integer ID: 9665
5% β-Mercaptoethanol
dash of Bromophenol blue just so it turns sky blue
30% Glycerol
10% SDS
250 mM Tris-Cl, pH 6.8