Jun 26, 2020
Water Filtration with Peristaltic Pump or Syringe & DNA Preservation
- 1University of Tennessee, Knoxville
- Engel Lab at Tennessee
Protocol Citation: Annette S Engel, Audrey Paterson, Hannah Rigoni 2020. Water Filtration with Peristaltic Pump or Syringe & DNA Preservation. protocols.io https://dx.doi.org/10.17504/protocols.io.bhxyj7pw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol in our group and it is working.
Created: June 26, 2020
Last Modified: July 10, 2020
Protocol Integer ID: 38616
Keywords: field sampling, filtration, water, eDNA,
Abstract
Cells, particulates, and fine sediment (i.e., clay) in water are retained on filters during filtration. Filtered water can be collected for geochemical analyses and nucleic acids (referred to as environmental DNA or eDNA) can be extracted from the filters. This protocol describes water filtration with a 0.22 mm Sterivex (Millipore) filter cartridge by using a peristaltic pump or manually with a syringe, and the preservation of filtered material collected on the filter membrane using CTAB.
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