Feb 16, 2026
VTA-PnO article
- 1Leibniz Institute für Neuroniologie
Collection Citation: Cristian González-Cabrera 2026. VTA-PnO article. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l6epmrgqe/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 16, 2026
Last Modified: February 16, 2026
Collection Integer ID: 243396
Keywords: pontine gaba pathway, backward locomotion, distal inhibition, vta
Abstract
This collection contains all experimental protocols associated with the manuscript “A VTA–pontine GABA pathway drives backward locomotion via local and distal inhibition”.
Files
Protocol
Name
Open-Field Video Tracking and Locomotion Classification (DeepLabCut Pipeline)
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Three-Dimensional Neuron Reconstruction from Confocal Z-Stacks (Neurolucida)
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Whole-Brain Axonal Projection Quantification Using ABBA and QuPath
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
In Vivo Fiber Photometry Recording With Concurrent Optogenetic Stimulation
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Open-Field Locomotion Assay with Optogenetic Stimulation
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Rotarod Forced Locomotion Assay During Chronic Electrophysiological Recording
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Juxtacellular Recording and Single-Cell Labeling of VTA Neurons
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Immunofluorescence on Free-Floating Brain Sections
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Acute Midbrain Slice Preparation
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Ex Vivo Whole-Cell Patch Clamp Recording and Pharmacological Isolation of Light-Evoked GABAA Currents
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
VTA and PnO Viral Injections With Optic Fiber Implantation
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
VTA and PnO Viral Injections With Optrode Implantation
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
Stereotaxic Viral Injections Into VTA and PnO (No Implant)
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie
Protocol
Name
In Vivo Opto-Tagging and Extracellular Recording (Acute and Chronic)
Version 1
Created by
Cristian González-CabreraLeibniz Institute für Neuroniologie