Apr 25, 2024
Von Stosch (VS) enriched seawater medium solution
- 1School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3FX, United Kingdom
Protocol Citation: Anton Kuech 2024. Von Stosch (VS) enriched seawater medium solution. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn77emv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2022
Last Modified: April 25, 2024
Protocol Integer ID: 57899
Keywords: Macroalgae, Seaweed cultivation, Enriched seawater medium, Cultivation medium, von Stosch
Funders Acknowledgements:
Natural Environment Research Council
Grant ID: NE/S007377/1
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Abstract
This protocol outlines the steps required to prepare von Stosch (VS) enriched seawater medium modified according to Guiry & Cunningham (1984).
CITATION
Protocol materials
Vitamin B12Fisher ScientificCatalog #68-19-9
MilliQ water
MilliQ water
Titriplex® III solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #1099920001
Sodium GlycerophosphateCatalog # S8041-100GM
Iron(II) sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #215422
Manganese(II) chloride tetrahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M3634
BiotinP212121
Thiamine HClP212121
MilliQ water
Potassium iodide
Vitamin B12Fisher ScientificCatalog #68-19-9
MilliQ water
Before start
It is important to follow the order of chemicals added as shown in the protocol.
Vitamin B12 stock solution
Vitamin B12 stock solution
5 mg Vitamin B12Sigma AldrichCatalog #68-19-9 in 500 mL MilliQ waterSigma Aldrich
Note
The vitamin B12 stock solution has to be prepared separately to the primary solution and should be stored in the fridge.
Primary solution
Primary solution
In 4 L MilliQ waterSigma Aldrich dissolve:
1.86 g Titriplex® III solutionSigma AldrichCatalog #1099920001 (Na₂-EDTA 2 H₂O)
21.26 g Sodium GlycerophosphateSigma AldrichCatalog # S8041-100GM
0.139 g Iron(II) sulfate heptahydrateSigma AldrichCatalog #215422
0.98 g Manganese(II) chloride tetrahydrateSigma AldrichCatalog #M3634
0.05 g BiotinSigma Aldrich
0.1 g Thiamine HClSigma Aldrich
20 mL Vitamin B12 stock solution (5 mg in 500 mL MilliQ waterSigma Aldrich go to step #1 )
10 mg Potassium iodideSigma Aldrich
Pasteurise for 02:00:00 at 99 °C
Storage
Storage
Store VS solution in Duran bottles in a cold and dark setting at 10 °C .
Equipment
Duran® laboratory bottles, with caps
NAME
Glass bottle
TYPE
Duran
BRAND
Z305197-10EA
SKU
500 ml
SPECIFICATIONS
Application
Application
Culturing medium is created by adding 200 mL VS to 10 L sterile seawater
Note
Add VS solution to seawater only after it has cooled down.
Citations
M. D. Guiry & E. M. Cunningham. Photoperiodic and temperature responses in the reproduction of north-eastern Atlantic Gigartina acicularis (Rhodophyta: Gigartinales)
https://doi.org/10.2216/i0031-8884-23-3-357.1