Apr 25, 2024

Public workspaceVon Stosch (VS) enriched seawater medium solution

DOI
https://dx.doi.org/10.17504/protocols.io.14egn77emv5d/v1
  • Anton Kuech1
  • 1School of Biological Sciences, University of Aberdeen, Aberdeen AB24 3FX, United Kingdom
Anton Kuech
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DOI: https://dx.doi.org/10.17504/protocols.io.14egn77emv5d/v1
Protocol Citation: Anton Kuech 2024. Von Stosch (VS) enriched seawater medium solution. protocols.io https://dx.doi.org/10.17504/protocols.io.14egn77emv5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2022
Last Modified: April 25, 2024
Protocol Integer ID: 57899
Keywords: Macroalgae, Seaweed cultivation, Enriched seawater medium, Cultivation medium, von Stosch, enriched seawater medium solution, enriched seawater medium, von stosch, seawater medium solution this protocol, seawater medium, seawater, medium solution, medium solution this protocol
Funders Acknowledgements:
Natural Environment Research Council
Grant ID: NE/S007377/1
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Abstract
This protocol outlines the steps required to prepare von Stosch (VS) enriched seawater medium modified according to Guiry & Cunningham (1984).
Citation
M. D. Guiry & E. M. Cunningham (1984). Photoperiodic and temperature responses in the reproduction of north-eastern Atlantic Gigartina acicularis (Rhodophyta: Gigartinales). Phycologia.
https://doi.org/10.2216/i0031-8884-23-3-357.1
LINK

Protocol materials
ReagentVitamin B12Fisher ScientificCatalog #68-19-9
ReagentMilliQ water
ReagentTitriplex® III solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #1099920001
ReagentSodium GlycerophosphateCatalog # S8041-100GM
ReagentIron(II) sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #215422
ReagentManganese(II) chloride tetrahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M3634
ReagentBiotinP212121
ReagentThiamine HClP212121
ReagentPotassium iodide
Before start
It is important to follow the order of chemicals added as shown in the protocol.
Vitamin B12 stock solution
Amount5 mg ReagentVitamin B12Fisher ScientificCatalog #68-19-9 in Amount500 mL ReagentMilliQ water
Note
The vitamin B12 stock solution has to be prepared separately to the primary solution and should be stored in the fridge.

Primary solution
In Amount4 L ReagentMilliQ water dissolve:
Amount1.86 g ReagentTitriplex® III solutionMerck MilliporeSigma (Sigma-Aldrich)Catalog #1099920001 (Na₂-EDTA 2 H₂O)
Amount21.26 g ReagentSodium GlycerophosphateCatalog # S8041-100GM
Amount0.139 g ReagentIron(II) sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #215422
Amount0.98 g ReagentManganese(II) chloride tetrahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M3634
Amount0.05 g ReagentBiotinP212121
Amount0.1 g ReagentThiamine HClP212121
Amount20 mL Vitamin B12 stock solution (Amount5 mg in Amount500 mL ReagentMilliQ water Go to )
Amount10 mg ReagentPotassium iodide
Pasteurise for Duration02:00:00 at Temperature99 °C
Storage
Store VS solution in Duran bottles in a cold and dark setting at Temperature10 °C .
Equipment
Duran® laboratory bottles, with caps
NAME
Glass bottle
TYPE
Duran
BRAND
Z305197-10EA
SKU
500 ml
SPECIFICATIONS

Application
Culturing medium is created by adding Amount200 mL VS to Amount10 L sterile seawater
Note
Add VS solution to seawater only after it has cooled down.

Citations
M. D. Guiry & E. M. Cunningham. Photoperiodic and temperature responses in the reproduction of north-eastern Atlantic Gigartina acicularis (Rhodophyta: Gigartinales)
https://doi.org/10.2216/i0031-8884-23-3-357.1