Oct 06, 2019

Public workspaceVirus titration

PLOS One
DOI
https://dx.doi.org/10.17504/protocols.io.7zhhp36
  • Norfitriah Mohamed Sohaimi1,
  • Mohd Hair Bejo1,
  • Abdul Rahman Omar1,
  • Aini Ideris1,
  • Nurulfiza Mat Isa2
  • 1Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.;
  • 2Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Norfitriah Mohamed Sohaimi
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DOI: https://dx.doi.org/10.17504/protocols.io.7zhhp36
External link: https://doi.org/10.1371/journal.pone.0225863
Protocol Citation: Norfitriah Mohamed Sohaimi, Mohd Hair Bejo, Abdul Rahman Omar, Aini Ideris, Nurulfiza Mat Isa 2019. Virus titration. protocols.io https://dx.doi.org/10.17504/protocols.io.7zhhp36
Manuscript citation:
Sohaimi NM, Bejo MH, Omar AR, Ideris A, Isa NM (2019) Molecular characterization of fowl adenovirus isolate of Malaysia attenuated in chicken embryo liver cells and its pathogenicity and immunogenicity in chickens. PLoS ONE 14(12): e0225863. doi: 10.1371/journal.pone.0225863
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 06, 2019
Last Modified: October 06, 2019
Protocol Integer ID: 28425
Materials
MATERIALS
Reagent96-well flat-bottom tissue culture plates with lidsCorningCatalog #3596
Viral supernatant from passage 5 (CEL5), CEL10, CEL15, CEL20, CEL25, CEL30 and CEL35 were determined for titration by 50% tissue culture infective dose (TCID50) as described by Reed and Muench [1938]. Supernatant was diluted for 10 folds serial dilution and inoculated onto fresh CEL cells in 96 wells plate and monitored daily for CPE. Virus titre was calculated and expressed as TCID50/mL.
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