Oct 28, 2020

Public workspaceVirION 2

DOI
dx.doi.org/10.17504/protocols.io.6q9hdz6
  • 1The Ohio State University;
  • 2University of Exeter
  • Sullivan Lab
  • Temperton Lab
Natalie Solonenko
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DOI: dx.doi.org/10.17504/protocols.io.6q9hdz6
External link: https://doi.org/10.7717/peerj.11088
Collection Citation: Marie Burris, Ben Temperton, Natalie Solonenko 2020. VirION 2. protocols.io https://dx.doi.org/10.17504/protocols.io.6q9hdz6
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 22, 2019
Last Modified: October 28, 2020
Collection Integer ID: 27137
Abstract
This collection contains protocols for the extraction and preparation of DNA for long-read work and library amplification of long reads from microbial or viral DNA.
Safety warnings
Always use phenol:chloroform and chloroform in a fume hood.
Files
Protocol
Icon representing the file Ampure bead clean up for high molecular weight DNA
Name
Ampure bead clean up for high molecular weight DNA
Version 1
, OSU
Marie BurrisOSU
Protocol
Icon representing the file DNA extraction for HMW DNA
Name
DNA extraction for HMW DNA
Version 1
, The Ohio State University
Natalie SolonenkoThe Ohio State University
Protocol
Icon representing the file Preparation of extracted DNA for long-read library prep
Name
Preparation of extracted DNA for long-read library prep
Version 1
, The Ohio State University
Natalie SolonenkoThe Ohio State University
Protocol
Icon representing the file Long Read Viromics Amplification Library Preparation (VirION 2)
Name
Long Read Viromics Amplification Library Preparation (VirION 2)
Version 1
, OSU
Marie BurrisOSU