Summary:Mice with catheters implanted in the jugular vein (infusions) and carotid artery (sampling) are used for this procedure (V3002). The hyperinsulinemic hypoglycemic clamp involves a constant rate insulin infusion with a fall in blood glucose that is controlled by feed back from regular glucose measurements. Blood glucose is then clamped at a hypoglycemic level. The hypoglycemic clamp is used to test hypoglycemic counterregulation and the functionality of the hypothalamic-pituitary-adrenal axis.