May 05, 2026

van Veen et al, 2026

DOI
https://dx.doi.org/10.17504/protocols.io.4r3l29p3xv1y/v1
  • 1KU Leuven;
  • 2Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815
Sarah van Veen
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DOI: https://dx.doi.org/10.17504/protocols.io.4r3l29p3xv1y/v1
Collection Citation: Sarah van Veen 2026. van Veen et al, 2026. protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l29p3xv1y/v1
License: This is an open access  collection  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 04, 2025
Last Modified: May 05, 2026
Collection  Integer ID: 119519
Keywords: ASAPCRN, paper van veen et al, protocol, van veen et al
Funders Acknowledgements:
Fonds Wetenschappelijk Onderzoek (FWO)
Grant ID: G011424N
Aligning Science Across Parkinson’s
Grant ID: ASAP-020607
Aligning Science Across Parkinson's
Grant ID: ASAP-000458
Abstract
Protocols related to the paper van Veen et al, 2026.
Files
Protocol
Icon representing the file Generation of stable cell lines using Lentiviral vectors
Name
Generation of stable cell lines using Lentiviral vectors
Version 1
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Image of Joris Van Asselberghs, KU Leuven
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RT-qPCR (cell lines)
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Name
Fluorescently labeled polyamine uptake (via flow cytometry)
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Fluorescently labeled polyamine uptake (via confocal microscopy)
Version 1
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Lentiviral vector production and primary astrocyte transduction
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Protocol
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RNA isolation and cDNA synthesis
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RT-qPCR (primary cells)
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Protocol
Name
Astrocyte Isolation and Transfection for Astrocytes/Neuron co-culture assay
Version 1
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Sarah van VeenKU Leuven
Protocol
Name
Astrocyte Isolation and ACM Production
Version 1
Created by
Image of Justin T Savage, Duke University
Justin T SavageDuke University
Protocol
Name
Astrocyte morphology assessment in astrocyte-neuron co-cultures
Version 1
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Protocol
Name
Glia-Free Cortical Neuron Culture
Version 1
Created by
Image of Justin T Savage, Duke University
Justin T SavageDuke University
Protocol
Name
Glia-Free Cortical Neuronal Feeding Schedule - Neuronal Morphology
Version 1
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Protocol
Name
Glia-Free Cortical Neuronal Feeding Schedule - Synapse Formation
Version 1
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Protocol
Name
Multiplexed immunofluorescence and RNA-FISH
Version 1
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Sarah van VeenKU Leuven
Protocol
Name
Postnatal astrocyte labeling by electroporation (PALE)
Version 1
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Protocol
Name
Expression and purification of ATP13A4 from Saccharomyces cerevisiae
Version 1
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Protocol
Name
Purification of ATP13A4 from HEK293T cells
Version 1
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Protocol
Name
Protein quantification by SDS-PAGE and Coomassie staining
Version 1
Created by
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Protocol
Name
Measurement of ATP13A4 ATPase activity using ADP-Glo Max Assay
Version 1
Created by
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Sarah van VeenKU Leuven
Protocol
Name
Auto-(de)phosphorylation assay
Version 1
Created by
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Protocol
Name
Preparation of total cell lysates
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Immunoblotting
Version 1
Created by
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Protocol
Name
Immunocytochemistry - subcellular localization of ATP13A4
Version 1
Created by
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Protocol
Name
Genotyping Atp13a4(KO) mice
Version 1
Created by
Image of Sarah van Veen, KU Leuven
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Protocol
Name
Brain slice preparation for electrophysiology recording
Version 1
Created by
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Protocol
Name
Metabolomic analysis of polyamines
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Synapse staining - mouse brain sections
Version 1
Created by
Image of Sarah van Veen, KU Leuven
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Protocol
Name
in vivo TurboID
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
IHC staining (DAB) of Iba1 and Gfap
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Collection of mouse cerebrospinal fluid from the cisterna magna
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Brain dissection protocol
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Facial vein blood collection
Version 1
Created by
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Sarah van VeenKU Leuven
Protocol
Name
DigiGait: Quantitative Gait Analysis in Mice
Version 1
Created by
Image of Sarah van Veen, KU Leuven
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Protocol
Name
Three-Chamber Social Preference Test in Mice
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
PTZ Seizure Challenge: Assessment of Seizure Sensitivity in Mice
Version 1
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Protocol
Name
Accelerating Rotarod: Motor Coordination and Balance Assessment in Mice
Version 1
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Sarah van VeenKU Leuven
Protocol
Name
Morris Water Maze: Spatial Learning and Memory Assessment in Mice
Version 1
Created by
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Protocol
Name
Open Field Exploration: Locomotor Activity and Anxiety Assessment in Mice
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
24-Hour Home Cage Activity Monitoring in Mice
Version 1
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Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Developmental milestones assessment in newborn mice (P1–P21)
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven
Protocol
Name
Isolation and culture of primary mouse astrocytes and neurons
Version 1
Created by
Image of Sarah van Veen, KU Leuven
Sarah van VeenKU Leuven