Jan 11, 2024

Public workspaceUV decontamination of reagents/buffers V.1

DOI
https://dx.doi.org/10.17504/protocols.io.e6nvwdn5dlmk/v1
  • Elena Essel1,
  • Matthias Meyer1,
  • Merlin Szymanski2
  • 1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany;
  • 2Max Planck Institute for Evolutionary Anthropology
  • MPI EVA Ancient DNA Core Unit
Elena Essel
Icon indicating open access to content
QR code linking to this content
DOI: https://dx.doi.org/10.17504/protocols.io.e6nvwdn5dlmk/v1
Document Citation: Elena Essel, Matthias Meyer, Merlin Szymanski 2024. UV decontamination of reagents/buffers. protocols.io https://dx.doi.org/10.17504/protocols.io.e6nvwdn5dlmk/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: December 29, 2023
Last Modified: April 10, 2024
Document Integer ID: 92814
Keywords: ancient dna cleanroom by uv treatment, uv decontamination of reagent, dna contamination of reagent, uv decontamination, reducing dna contamination, ancient dna cleanroom, uv treatment, contamination, dna, reagent, buffers protocol
Funders Acknowledgements:
Max Planck Society
Abstract
Protocol for reducing DNA contamination of reagents and buffers used in the ancient DNA cleanroom by UV treatment.
Note

The effectiveness of UV treatment for DNA decontamination strongly depends on the permeability of material for UV light. Falcon tubes, plastic bottles and regular glass bottles absorb UV light and are therefore poorly suitable for UV-based DNA decontamination. Thus, UV treatment of reagents and buffers should be performed in quartz glass bottles. Perform UV treatment only if indicated in the respective protocols/documents for reagent usage or preparation.

Materials
MaterialSupplierCat. no. Decontamination *
Quartz glass bottles with plastic screw capQuarzglas Komponenten und Service (custom produced) -Bleach + UV
250 ml squared plastic bottlesVWR391-0629-
50 ml Falcon tubesGreiner Bio-one210261-
* See documents in the Appendix for decontamination instructions
Equipment

  • UV Crosslinker (e.g., Bio-Link UV Crosslinker, Labortechnik, cat. no. 110.0079)
  • aluminum foil (e.g., Roth, cat. no. AA76.1)


Procedure

1. For decontamination of reagents/buffers, pour the reagent/buffer from the stock bottle into a clean quartz glass bottle. Make sure the stock bottle is not touching the quartz glass bottle while pouring.

Note
[Labeling]
Label the quartz glass bottle with the name of the reagent, date and your initials.

2. Close the quartz glass bottle properly to avoid any leaking during UV treatment.

3. UV treat the glass bottles in the UV Crosslinker set to 7.000 J (approximate time of irradiation is 40 min).

4. Refill the reagent into a bottle that is appropriate for long-term storage (e.g. 50 ml Falcon tube, 250 ml squared bottle).

Note
[Labeling]
Label the storage bottle with the name of the reagent, date (JJJJMMDD), 'UV treated' and your initials.


Note
[Note]
Never store reagents/buffers in the quartz glass bottle, because the lids are not closing tightly. Never pipette out of the quartz glass bottle.

5. Bleach and UV treat the silica bottle following the procedure described in the Appendix before putting it back into the stock.

Appendix
Document
NAME
Bleaching and UV decontamination of materials
CREATED BY
Elena Essel