Jan 10, 2024

Public workspaceUV decontamination of materials V.1

DOI
https://dx.doi.org/10.17504/protocols.io.5qpvo31y9v4o/v1
  • Elena Essel1
  • 1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, D-04103 Leipzig, Germany
  • MPI EVA Ancient DNA Core Unit
Elena Essel
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DOI: https://dx.doi.org/10.17504/protocols.io.5qpvo31y9v4o/v1
Document Citation: Elena Essel 2024. UV decontamination of materials . protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvo31y9v4o/v1
License: This is an open access document distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: December 29, 2023
Last Modified: April 11, 2024
Document Integer ID: 92816
Keywords: ancient dna cleanroom by uv treatment, uv decontamination of materials protocol, uv decontamination, uv decontamination of material, reducing dna contamination, dna contamination on tube, ancient dna cleanroom, uv treatment, dna, contamination, materials protocol, bottle
Funders Acknowledgements:
Max Planck Society
Abstract
Protocol for reducing DNA contamination on tubes, bottles, zip lock bags and other reaction vessels and containers used in the ancient DNA cleanroom by UV treatment.
Troubleshooting
Note

The effectiveness of UV treatment for DNA decontamination strongly depends on the permeability of material for UV light.

Materials
ConsumablesSupplier
Tubes, storage vessel/container, zip-lock bag, other itemsvarious
Equipment

  • UV Crosslinker (e.g., Bio-Link UV Crosslinker, Labortechnik, cat. no. 110.0079)
  • aluminum foil (e.g., Roth, cat. no. AA76.1)

Protocol

Note
Always use fresh gloves for handling the materials you want to decontaminate before and especially after UV treatment to avoid any additional contamination being introduced to the materials.

  1. If the materials you want to UV treat can be closed (e.g. Eppendorf tubes) close them to avoid contamination after treatment.
  2. Place the materials on a piece of aluminum foil inside the UV cross linker.
  3. UV treat equipment twice in Crosslinker set to 7.000 J (approximate time of irradiation is 40 min per round).
  4. Store cleaned and UV treated equipment in an appropriate, clean storage container.

Note
[Labeling]
Label the storage container with content, date and your initials.