Aug 09, 2024

Public workspaceURMC TriState SenNet TMC Mouse Sacrifice and Organ Harvest

DOI
dx.doi.org/10.17504/protocols.io.rm7vzj2j8lx1/v1
  • 1Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY
  • TriState SenNet
Gagandeep Kaur
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.rm7vzj2j8lx1/v1
Protocol Citation: Gagandeep Kaur, Irfan Rahman 2024. URMC TriState SenNet TMC Mouse Sacrifice and Organ Harvest. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzj2j8lx1/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 09, 2024
Last Modified: August 09, 2024
Protocol Integer ID: 105002
Keywords: Mouse handling, Sacrifice, Lung harvest
Funders Acknowledgements:
TriState SenNet
Grant ID: Grant ID: U54 AG075931
Disclaimer
The authors have no conflict of interest to declare.
Abstract
This protocol outlines the method used to sacrifice and harvest the mouse lung tissue to perform downstream analyses for the TriState SenNet TMC Biospecimen Core at the University of Rochester, as part of the Cellular Senescence Network Program (SenNet).
Care was taken to reduce the pain and distress caused to the mice by performing all the procedures after anesthetising the mice.
Materials
1. Sodium Pentabarbital (Cat# NDC 25021-676-20, Sagent)
2. Insulin Syringes
3. Saline
4. 3mL Syringes
5. Eppendorf tubes
6. Scissors
7. 1X PBS

Sodium Pentobarbitol ( 60-90 mg/kg of body weight) was administered intraperitoneally to anaesthetize the mice prior to organ harvest.
After 5 min of drug administration, the animal response was assessed by pinching the mouse's foot with forceps and observing the withdrawal response/pedal reflex.
Upon successful anesthetization, the animals abdomen and chest was cut open and mice was exsanguinated by cardiac puncture.
Thereafter, the thoracic cavity was cut open to harvest the mouse lung.
The harvested organ was stored in PBS and kept TemperatureOn ice to perform future assays/ assessments.