Aug 07, 2024
URMC TriState SenNet TMC Mouse Donor Selection Criteria
- 1Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY;
- 2University of Rochester
- TriState SenNet
Protocol Citation: Gagandeep Kaur, Irfan Rahman 2024. URMC TriState SenNet TMC Mouse Donor Selection Criteria. protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gpz2wdlzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: August 07, 2024
Last Modified: August 07, 2024
Protocol Integer ID: 104913
Keywords: Mouse , Inclusion/exclusion criteria, Cellular senescence
Funders Acknowledgements:
TriState SenNet
Grant ID: U54 AG075931
Disclaimer
The authors have no conflict of interest to declare.
Abstract
This document outlines the required criteria for donor inclusion of mouse lung specimens in the TriState SenNet TMC Biospecimen Core at the University of Rochester, as part of the Cellular Senescence Network Program (SenNet).
The study intends to outline the effect of age on the cellular senescence in mice and intends to describe the translatability of obtained results.
Inclusion Criteria
Inclusion Criteria
Age: Adult mice. Two age ranges:
Young Adults : 2-6 months
Old adults (geriatric) : 18-24 months
Sex: Both sexes were included.
Strain: C57Bl/6J mice were procured from Jackson Laboratory. Mice were housed for 1 week at the University of Rochester Vivarium with a 12-hour light/12-hour dark cycle.
Health Status: No known health issues or abnormalities approved by the Veterinary staff at the University of Rochester Vivarium facilities.
Exclusion Criteria
Exclusion Criteria
Infection/Disease: The animals were housed in a Helicobacter- and pathogen-free facility. Necessary precautions (use of microisolator technique, one-way MIT rooms) were taken to ensure that the study animals did not have any infection or disease state prior to conducting of experiments.