Dec 19, 2022
UPitt TriState SenNet TMC Lung Procurement
- John Sembrat1,
- Alison Morris1,
- koenigshoffm 1,
- Oliver Eickelberg1
- 1Division of Pulmonary and Critical Care Medicine. Department of Medicine. School of Medicine University of Pittsburgh
- TriState SenNet
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: John Sembrat, Alison Morris, koenigshoffm , Oliver Eickelberg 2022. UPitt TriState SenNet TMC Lung Procurement. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbyr83vpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 18, 2022
Last Modified: December 19, 2022
Protocol Integer ID: 74181
Keywords: upitt tristate sennet tmc lung procurement this document, upitt tristate sennet tmc lung procurement, tristate sennet tmc biospecimen core at the university, tristate sennet tmc biospecimen core, arrival at the tristate sennet tmc biospecimen core, upitt tristate sennet tmc, part of the cellular senescence network program, cellular senescence network program, sennet, request for specimen procurement, specimen procurement, lung
Funders Acknowledgements:
TriState SenNET (Lung and Heart) Tissue Map and Atlas consortium - NIA
Grant ID: U54AG075931
Abstract
This document outlines the request for specimen procurement before arrival at the TriState SenNet TMC Biospecimen Core at the University of Pittsburgh, as part of the Cellular Senescence Network Program (SenNet).
Troubleshooting
Requested processing protocol for Donation following Brain Death Declaration (DBD)
Organ Procurement Organization Recovery Team prepares for organ recovery as consistent with organ transplant protocol with cross clamp and in situ cold flush body cooling with minimal warm ischemic time.
Includes flush with Perfadex or similar organ transplant buffer, until solution runs clear
Complete a minimum back-table lung flush with transplant buffer of 500ml into the pulmonary artery and 500ml into the pulmonary vein.
Do not flush with saline.
Do not wrap in surgical towel.
Staple, clamp, or tie the trachea closed prior to shipping.
Place Lung(s) in a Specimen Bag and submerge with chilled Perfadex or similar. Triple bag the tissue.
Requested processing protocol for Donation following Cardiac Death /Arrest(DCD)
Organ Procurement Organization / Recovery Team prepares for organ recovery as quickly following Cardiac Arrest while in comfort care as authorized by next of kin.
Remainder of organ / tissue recovery should be as consistent with organ transplant protocol (section above) as possible.
Includes organ flush with Perfadex or similar organ transplant buffer until solution runs clear.
All warm ischemic time is to be minimized and reported. Tissues may be accepted up to 6 hours WIT. Other WIT may be evaluated depending of the circumstances.