Cattle's blood component is compressed using centrifuge (5000 rpm for 10 minutes), then supernatant (erythrocytes) collected. Erythrocytes with a ratio of 3:1 (serum:supernatant), treated with initial mixing each with HCl (0.5 M) NaOH (0.5 M), and 30% H2O2with an initial ratio of 1:1 (v/v). Each variable then treated by mixing SnCl2(1 M, 1:1 v/v), oleic acid (2:1 v/v), and O2(g) (0.5 L/m, 60s). The best sample then purified by heat about 800oC for 120 minutes.The highest calculated total iron mass is 240000 μg/100 ml (NaOH + oleic acid). Sample purification has increased the iron concentration up to 46.30% (m/m%). .justify:after { content: ""; display:inline-block; width: 100%; }