Aug 14, 2020
University College Dublin (UCD) Zebrafish Facility Environmental Parameters: 2019
- Grace Kennedy1,2,
- Husvinee Sundaramurthi1,2,3,4,
- UCD Biomedical Facility Staff 5,
- Breandán Kennedy 1,2
- 1UCD Conway Institute, University College Dublin, Dublin, Ireland;
- 2UCD School of Biomolecular & Biomedical Science, University College Dublin, Dublin, Ireland;
- 3UCD School of Medicine, University College Dublin, Dublin, Ireland;
- 4Systems Biology Ireland, University College Dublin, Dublin, Ireland;
- 5Biomedical Facility, University College Dublin, Dublin, Ireland
Document Citation: Grace Kennedy, Husvinee Sundaramurthi, UCD Biomedical Facility Staff , Breandán Kennedy 2020. University College Dublin (UCD) Zebrafish Facility Environmental Parameters: 2019. protocols.io https://dx.doi.org/10.17504/protocols.io.bjqikmue
License: This is an open access document distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Created: August 14, 2020
Last Modified: August 14, 2020
Document Integer ID: 40426
Keywords: Zebrafish Facility, Environmental parameters,
Abstract
Environmental factors and animal husbandry conditions are known to have implications in outcome of study results. To this end, environmental parameters (i.e. temperature, pH and conductivity) of our zebrafish facility are constantly monitored to ensure constant/limited variations to be introduced in the environment, and hence affecting our research studies. Our facility com. prises of a recirculating water system with filtration, sterilization and automatic adjustment of water parameters (pH, conductivity, temperature) with a capacity of over 4,000 litres of water, it houses over 20,000 adult fish in 1-, 3-, and 10-litre tanks. A separate nursery system houses up to 12,000 larvae aged from 6 to 30 days post fertilisation.
We have included information of our facility and key parameters measured for our zebrafish lines housed in University College Dublin for the year 2019. These conditions are applicable to all recent peer-reviewed articles published by our group.
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