Jun 03, 2020
Version 4
UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary V.4
- 1University of North Carolina at Chapel Hill
External link: https://doi.org/10.7554/eLife.83652
Protocol Citation: Michelle Altemara 2020. UNC Chapel Hill Zebrafish Aquaculture Core (ZAC) Environmental Summary. protocols.io https://dx.doi.org/10.17504/protocols.io.bg3jjykn
Manuscript citation:
Buglak DB, Bougaran P, Kulikauskas MR, Liu Z, Monaghan-Benson E, Gold AL, Marvin AP, Burciu A, Tanke NT, Oatley M, Ricketts SN, Kinghorn K, Johnson BN, Shiau CE, Rogers S, Guilluy C, Bautch VL, Nuclear SUN1 stabilizes endothelial cell junctions via microtubules to regulate blood vessel formation. eLife doi: 10.7554/eLife.83652
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 03, 2020
Last Modified: June 03, 2020
Protocol Integer ID: 37707
Keywords: zebrafish, Danio rerio, reproducibility, husbandry, fish, model
Abstract
Zebrafish (Danio rerio) are a popular animal model used in a variety of research areas. As with all animal models, husbandry conditions, including environmental parameters, nutrition, and exposure to pathogens can affect research results. Additionly, zebrafish are tolerant of a wide range of environmental parameters, which has led to wide variation in husbandry practices across facilities around the world. The lack of standard conditions across zebrafish laboratories may play a role in irreproducible experiments. Therefore, annual summaries of these conditions have been compiled for the Zebrafish Aquaculture Core (ZAC) facility at the University of North Carolina (UNC) Chapel Hill with the aim to have this data included in research articles published using our fish. As more institutions publish husbandry protocols, standard husbandry practices may emerge.
