Feb 13, 2026
UMN TMCs: Fresh Frozen Tissue Protocol
- BLS Histology .1
- 1Clinical and Translational Science Institute, University of Minnesota, Minneapolis, MN
- Cellular Senescence Network (SenNet) Method Development Community
Protocol Citation: BLS Histology . 2026. UMN TMCs: Fresh Frozen Tissue Protocol . protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1k9o2gr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 22, 2026
Last Modified: February 13, 2026
Protocol Integer ID: 239169
Keywords: fresh frozen tissue protocol, protocol from bls histology, bls histology, umn tmc, obtained tissue
Abstract
Protocol from BLS Histology at University of Minnesota for processing snap frozen prospective surgically obtained tissue.
Guidelines
This protocol required prior approval by the users' local Institutional Animal Care and Use Committee (IACUC), Institutional Review Board (IRB) or equivalent ethics committee(s), depending on the source of the tissue.
Materials
PBS buffer
Cryovials
Liquid nitrogen bath
Troubleshooting
Before start
Prepare a liquid nitrogen bath large enough to hold a cryovial
Fresh Frozen Tissue
Tissue is received from surgical team and cut to a smaller size, ~1g
Tissue is placed in PBS buffer
When ready to freeze sample, remove tissue from PBS buffer and tap off excess buffer with a kimwipe
Tissue is placed in appropriately labeled cryovial
Snap freeze the cryovial in liquid nitrogen and store at -80°C