Summary: One index of arterial function that is compromised to a varying degree in individuals with cardiovascular disease is vascular stiffness. This protocol can be utilized to analyze vascular function as an index for cardiovascular disease. This protocol directly measures the stress and strain on an arterial vessel as an index of vascular function.Modified from: Symons et. al. Arterioscler Thromb Vasc Biol. 2002 May 1;22(5):772-80.