May 10, 2019

Public workspaceU Mass - Glucose

DOI
https://dx.doi.org/10.17504/protocols.io.xw5fpg6
U Mass - Glucose
  • Jason Kim1
  • 1University of Massachusetts
Lili Liang
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DOI: https://dx.doi.org/10.17504/protocols.io.xw5fpg6
External link: https://mmpc.org/shared/document.aspx?id=159&docType=Protocol
Protocol Citation: Jason Kim 2019. U Mass - Glucose. protocols.io https://dx.doi.org/10.17504/protocols.io.xw5fpg6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 08, 2019
Last Modified: May 10, 2019
Protocol Integer ID: 20157
Keywords: Glucose, Obesity, Insulin resistance, Type 2 diabetes., using analox gm9 glucose analyzer, analox gm9 glucose analyzer, glucose summary, sample glucose, plasma glucose level, plasma glucose concentration, glucose oxidase, glucose, reaction between sample glucose, hyperglycemia, diabetes, insulin, insulin resistance, rate of oxygen uptake, oxygen uptake, obesity
Abstract
Summary:

This experiment measures blood or plasma glucose concentration using Analox GM9 Glucose Analyzer or GM7 Micro-Stat Rapid Multi-assay Analyzer. The measurement is based on the rate of oxygen uptake in the reaction between sample glucose and glucose oxidase. Plasma glucose levels are elevated (i.e., hyperglycemia) in obesity, insulin resistance, and type 2 diabetes.
Materials
MATERIALS
ReagentAnalox GM9 Glucose Analyzer AnaloxCatalog #GM9 Glucose Analyser
ReagentAnalox GM7 Microstat Analyzer AnaloxCatalog #GM7 Microstat Analyzer
ReagentGlucose ReagentAnaloxCatalog #GMRD-002A
ReagentGlucose Standard (144.1 mg/dl) AnaloxCatalog #GMRD-011
ReagentMicroman M10 Precision Pipet GilsonCatalog # F148501
Troubleshooting
Before start
Notes:

√ Freshly prepared blood or plasma samples are recommended. If storing samples, keep blood and plasma samples at –20º C or at -70ºC for long-term storage. Avoid freeze/thaw cycles.

√ Avoid using samples with gross hemolysis or lipemia.

√ Allow all reagents to come to room temperature before measurement.
Check waste and fill glucose reagent reservoir.
Prepare ~0.1ml of standard solution (~144 mg/dl).
Press “Enter”, and then “No” to cycle the Analox Analyzer.
When cycling is finished, wait for reading to return to ~0 mg/dl (±1mg/dl).
Enter calibration mode.
Using precision pipet, inject 10 µl of standard solution.
Rinse pipet with dH2O.
When value is reached, accept calibration.
When reading has returned to ~0 mg/dl, inject 10 µl of standard solution, and ensure that the value is ~144 mg/dl.
Repeat steps #5~#9 until calibration value is stable.
Check calibration by injecting 5 µl of standard and multiplying reported value by 2.
The final calculated value should be ~144 mg/dl.
Instrument is now ready for sample measurement. Use 5 or 10 µl of plasma sample for glucose concentration measurement.