Summary: This experiment provides the quantification of multiple hormones using multiplexed-Luminex technology based on beads containing specific antibodies. Sample matrices include plasma, serum, tissue culture supernatants, and tissue or cell lysates. Serum glucagon levels reflect glucagon secretion and pancreatic \u03b1-cell function. Serum glucagon levels regulate hepatic glucose production and glucose homeostasis. Serum glucagon levels are altered in obesity, insulin resistance, and counterregulatory stress response.