May 10, 2019
U Mass - Energy balance – food intake, energy expenditure, physical activity
- Jason Kim1
- 1University of Massachusetts
- Mouse Metabolic Phenotyping CentersTech. support email: info@mmpc.org
Protocol Citation: Jason Kim 2019. U Mass - Energy balance – food intake, energy expenditure, physical activity. protocols.io https://dx.doi.org/10.17504/protocols.io.xwwfpfe
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 08, 2019
Last Modified: May 10, 2019
Protocol Integer ID: 20150
Keywords: food intake, energy expenditure, physical activity
Abstract
Summary:
The TSE PhenoMaster/LabMaster Metabolic Cage system is used to measure indirect calorimetry, food/water intake, energy expenditure, and physical activity in individually housed awake mice. The experiment noninvasively measures VO² consumption and VCO² production rates in individual mice using metabolic chambers and calculates the respiratory exchange ratio (respiratory quotient) to reflect energy expenditure. Metabolic cage measurement is conducted continuously for 72 hours (3 days) to account for acclimation of mice housed in home cages during the study. With our high-speed Siemens O²/CO² sensing unit, indirect calorimetry measurements may be performed at a 20-min interval for a full 12-cage study.
Materials
MATERIALS
TSE Phenomaster Metabolic Cages TSECatalog #TSE Phenomaster Metabolic Cages
LabMaster Software TSECatalog #LabMaster Software
Mouse diets
Drinking water
Corn-cob bedding
Metabolic cage food and water baskets are calibrated.
Drink and food baskets are filled with water and appropriate diet.
Corncob bedding is added to the cages.
Mice are individually housed in metabolic cages and checked daily for access to food and water.
Software is set to collect data at selected interval for 3 consecutive days (72 hour measurement period).
Post-run data are exported and analyzed using Microsoft Excel program.
Mice should be assessed for body composition (whole body lean mass) prior to metabolic cage measurement in order to incorporate whole body lean mass data for energy expenditure calculation.
Metabolic cages require monitoring on a daily basis to ensure food and water are accessible to the mice.