May 10, 2019

Public workspaceU Mass - Cholesterol (Total)

DOI
dx.doi.org/10.17504/protocols.io.xucfnsw
  • Jason Kim1
  • 1University of Massachusetts
  • Mouse Metabolic Phenotyping Centers
    Tech. support email: info@mmpc.org
Lili Liang
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DOI: dx.doi.org/10.17504/protocols.io.xucfnsw
External link: https://mmpc.org/shared/document.aspx?id=169&docType=Protocol
Protocol Citation: Jason Kim 2019. U Mass - Cholesterol (Total). protocols.io https://dx.doi.org/10.17504/protocols.io.xucfnsw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 06, 2019
Last Modified: May 10, 2019
Protocol Integer ID: 20068
Keywords: total cholesterol, cardiovascular disease
Abstract
Summary:

This experiment involves a spectrophotometric measurement using Roche Cobas Clinical Chemistry Analyzer. Serum levels of total cholesterol reflect cholesterol metabolism and are associated with cardiovascular disease.
Materials
MATERIALS
ReagentCholesterol Plus 2nd gen RocheCatalog #05401682 190
ReagentCalibrator f.a.s. Lipids RocheCatalog #12172623 160
ReagentPrecinorm L RocheCatalog #10781827 122
ReagentPrecipath HDL/LDL-CRocheCatalog #11778552 122
ReagentNaCl Diluent 9%RocheCatalog #04774230 190
ReagentCleanerRocheCatalog #04774248 190
ReagentMicro Sample cupsRocheCatalog #11406680 001
ReagentNERL High Quality Water Fisher ScientificCatalog #9805
Note:

Roche, RRID:SCR_001326
Fisher Scientific, RRID:SCR_008452
Before start
Notes:

√ Try to use freshly prepared serum and plasma samples for this assay.

√ No dilution or treatment of the sample is required, but plasma samples should be centrifuged to remove any fibrin/fibrinogen clumps.

√ Samples should be stored at 2-8°C for 24 hours prior to analysis. For longer periods, store samples at -70°C, and avoid repeated freeze/thaw cycles.

√ A 50 l dead volume is required in addition to sample volume for multi-protein analysis (typically 1-5 l).
Perform daily quality control assessment of instrumentation before analysis.
Load each sample into a specialized micro-sample cup for the clinical chemistry analyzer.
Select Cholesterol (Total) test on display and run the analysis.
Collect and analyze the data.