Jul 24, 2024

Ty + AS + Thymidine Protocol

This protocol is a draft, published without a DOI.
  • 1UGA
Icon indicating open access to content
QR code linking to this content
Protocol CitationEleanor Harman 2024. Ty + AS + Thymidine Protocol. protocols.io https://dx.doi.org/
License: This is an open access  protocol  distributed under the terms of the  Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 23, 2024
Last Modified: July 24, 2024
Protocol  Integer ID: 103946
Keywords: thymidine protocol, protocol
Abstract
.
Media
Yeast Extract 1.5g/500mL
Tryptone 2.5g/500mL
Thymidine 150mg/500mL
Add 80% final volume of DI water to beaker
Add Yeast Extract, Tryptone, and Thymidine until fully dissolved
Adjust pH to 5.5
Autoclave on Liquid 20 minute cycle