Jul 24, 2024
Ty + AS + Thymidine Protocol
This protocol is a draft, published without a DOI.
- 1UGA
Protocol Citation: Eleanor Harman 2024. Ty + AS + Thymidine Protocol. protocols.io https://protocols.io/view/ty-as-thymidine-protocol-dhri354e
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 24, 2024
Last Modified: July 24, 2024
Protocol Integer ID: 103946
Abstract
.
Media
Media
Yeast Extract 1.5g/500mL
Tryptone 2.5g/500mL
Thymidine 150mg/500mL
Add 80% final volume of DI water to beaker
Add Yeast Extract, Tryptone, and Thymidine until fully dissolved
Adjust pH to 5.5
Autoclave on Liquid 20 minute cycle