Jun 01, 2023

Tween 60-esculin Agar (TE slant)

Tween 60-esculin Agar (TE slant)
  • 1Duke University
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Protocol CitationAmy Gladfelter 2023. Tween 60-esculin Agar (TE slant). protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvm73e5v3p/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 03, 2018
Last Modified: June 01, 2023
Protocol Integer ID: 17460
Keywords: ability of malassezia species, malassezia species, esculin agar, hydrolyzeesculin, malassezia, tween, species, te slant
Abstract
This media is used to determine ability of Malassezia species to hydrolyzeesculin and utilize Tween 60.
Guidelines
This media is used to determine ability of Malassezia species to hydrolyze esculin and utilize Tween 60.
peptone 10 g

glucose 10 g

yeast extract 2 g

ferric ammonium citrate 0.5 g

esculin 1 g

agar 15 g

Tween 60 5 mL

dH2O 1 L

Sterilize by autoclaving.