March, 2012; based on sample preparation guide v2, catalog # RS-930-1021, part # 15026495 Rev. A, August 2011Modifications: samples in PCR tubes (because a plate/seal will not be used, some numbered steps are skipped); include two rounds of poly-A selection (with two separate sets of beads); repeat clean-up step after PCR to remove additional adapters; elute with 1.5 ul less volume off of beads (to leave 1 ul behind, not 2.5 ul)