Jun 03, 2020

Public workspaceTris Buffered Saline (TBS)

DOI
dx.doi.org/10.17504/protocols.io.bfynjpve
  • 1University of Manitoba
Neilier Junior
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DOI: dx.doi.org/10.17504/protocols.io.bfynjpve
Protocol Citation: Neilier Junior 2020. Tris Buffered Saline (TBS). protocols.io https://dx.doi.org/10.17504/protocols.io.bfynjpve
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 05, 2020
Last Modified: June 03, 2020
Protocol Integer ID: 36590
Keywords: pH, Henderson-Hasselback, Biochemistry, Molecular Biology,
Abstract
A buffer solution has the function of resisting changes in pH even when adding powerful acids or bases. However, in the physiological environment the buffered system also provides cofactors for enzymatic reactions, critical salts and even essential nutrients for cells and tissues. Therefore, when trying to reproduce biological conditions in vitro, we must make the appropriate choice of the buffer. After all, it will provide the appropriate medium in which reactions will occur.
Materials
  • Distilled Water
  • ph Meter (sensitive)
  • Tris Base
  • NaCl
Safety warnings
Wear personal protective equipment: gloves, lab coat and mask.
Before start
Organize your workspace.

Make sure all solutions and equipment are available.
Tris Buffered Saline (TBS)
Tris Buffered Saline (TBS)

Note
Ph7.4
10 mM Tris
150 mM NaCl

Prepare Amount1 L of TBS by dissolving Amount1.21 g Tris Base and Amount8.7 g NaCl in Amount1 L of distilled water .

Adjust pH before use.
Note
Tris has a pKa of 8.3. Hence, the buffering capacity at Ph7.4 is minimal compared to phosphate buffer (pKa = 7.21)