Lipofectamine™ 2000 Transfection ProtocolDescriptionLipofectamine™ 2000 is a proprietary formulation for the transfection of nucleicacids (DNA and RNA) into eukaryotic cells providing the following advantages:• Highest transfection efficiency in many cell types and formats (e.g. 96-well).Refer to the Cell Lines database at www.invitrogen.com for a list of cell typessuccessfully transfected.• Nucleic acid-Lipofectamine™ 2000 complexes can be added directly to cells inculture medium, in the presence or absence of serum.• It is not necessary to remove complexes or change/add medium aftertransfection, but complexes may be removed after 4-6 hours.