This is our transfer protocol for the transfer of proteins using the Trans-Blot Turbo Transfer system from Bio-Rad. We use it with our single gel we have made based the One-Gel system by Ahn T et al (2001) (DOI:10.1006\/abio.2001.5038). It should work the same for Laemmli-based gel systems.We have achieved transfer from proteins ranging from 270 kDa to 26 kDa present in the same gel.The Turbo Transfer has been standarized in our lab since October 2016, therefore there are no publications yet using the method. Data using the Ahn et al gel system have been used in the following publications.Jim\u00e9nez-Soto LF, Haas R. The CagA toxin of Helicobacter pylori: abundant production but relatively low amount translocated. Sci Rep. 2016 Mar 17;6:23227.doi: 10.1038\/srep23227. PubMed PMID: 26983895; PubMed Central PMCID: PMC4794710.Jim\u00e9nez-Soto LF, Clausen S, Sprenger A, Ertl C, Haas R. Dynamics of the Cag-type IV secretion system of Helicobacter pylori as studied by bacterial co-infections. Cell Microbiol. 2013 Nov;15(11):1924-37. doi: 10.1111\/cmi.12166. PubMed PMID: 23844976.