Jun 04, 2025
Total protein quantification (BioRad, #5000006) for protein expression and cycloheximide assay
- 1NIMH
Protocol Citation: Jailyn Izu 2025. Total protein quantification (BioRad, #5000006) for protein expression and cycloheximide assay. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvjn895gk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 15, 2024
Last Modified: March 26, 2026
Protocol Integer ID: 99863
Keywords: total protein quantification, quantification of protein concentration, protein concentration, protein expression, cycloheximide, quantification, assay
Funders Acknowledgements:
National Institute of Mental Health
Grant ID: ZIA MH002982
National Institute of Mental Health
Grant ID: R01MH122471
National Institute of Neurological Disorders and Stroke
Grant ID: U01NS120836
Foundation for the National Institutes of Health
Grant ID: Deeda Blair Research Initiative for Disorders of the Brain
Abstract
Quantification of protein concentrations from cell lysates
Materials
- Pierce Bovine Serum Albumin Standard Ampules, 2 mg/mL (Thermo Scientific, #23209)
- M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, #78501)
- Borosilicate glass test tubes (Kimble Glass Inc., #735001275)
- Bio-Rad Protein Assay Dye Reagent Concentrate, 450 ml (BioRad, #5000006)
- PBS, pH 7.4 (Thermo Fisher Scientific, #10010023)
Quantify total protein in lysates