Protocol Citation: May Shen 2020. Total Protein Extraction from Plants. protocols.io https://protocols.io/view/total-protein-extraction-from-plants-bh94j98w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 07, 2020
Last Modified: July 07, 2020
Protocol Integer ID: 38940
Keywords: total protein extraction, sample preparation, western blotting, sample buffer, protein preparation,
Abstract
Total Protein Extraction from Plants
Materials
MATERIALS
2x Laemmli Sample BufferBioRad SciencesCatalog #1610737
Mortar and pestle
Before start
Heat up dry bath machine to 65 ℃.
New Sample Preparation
New Sample Preparation
20m
20m
0.1 g plant tissue +300 µL 2X sample buffer finely grounded.
Move into 1.5 ml eppendorf.
5m
65 °C dry bath for 00:15:00
15m
12000 rpm, 00:01:00
1m
Ready to load or store in -20 °C
Old sample thawing back
Old sample thawing back
11m
11m
65 °C dry bath for 00:10:00
10m
12000 rpm, 00:01:00
1m
Ready to load