Mar 06, 2020
Total Flavonoid Content (TFC)
- 1Hainan University
Protocol Citation: Jing Xu 2020. Total Flavonoid Content (TFC). protocols.io https://dx.doi.org/10.17504/protocols.io.bda6i2he
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 06, 2020
Last Modified: March 06, 2020
Protocol Integer ID: 33854
Abstract
The amounts of total flavonoids was quantified. The reaction mixture consisted of 1.0 ml of extract, 0.3 ml of 5% sodium nitrite and 4 ml of 60% ethanol. After 6 min, 0.3 ml of 10% aluminium nitrite was added. In the next 6 minutes, 4 ml of 1 M sodium hydroxide solution were added. Then, the volumetric tube was diluted to 10 ml. The absorbance was measured at 510 nm.TFC was calculated and expressed as rutin equivalents (RE). A calibration curve was constructed with different concentrations of rutin (15–75 mg/ml)as a standard.