The best way to determine TOP2 specific accessibility to chromatin is via etoposide treatment, which leads to a covalent cross-link between TOP2 proteins and DNA, that can only occur where TOP2 proteins have access to DNA. TOP2 cross-linking to DNA has previously been shown to require DNA accessibility and chromatin regulators that alter DNA accessibility (Dykhuizen and Hargreaves et al. Nature 2013, Miller et al. NSMB 2017).