Nov 29, 2023
Top agar: 0.7% w/v LB(Miller) agar
- HANNAH ZHU1
- 1macquarie uni, jaschke labs
Protocol Citation: HANNAH ZHU 2023. Top agar: 0.7% w/v LB(Miller) agar. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lyj2oelx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: November 28, 2023
Last Modified: November 29, 2023
Protocol Integer ID: 91547
Keywords: top agar for plaque assay experiment, plaque assay experiment, top agar, assay
Abstract
how to make Top agar for plaque assay experiment
Materials
Tryptone
Yeast extract
NaCl
Agar
Troubleshooting
Top agar: 0.7% w/v LB(Miller) agar
15m
Dissolve following components in 200 mL MilliQ water
| components | final concentration | for 200mls | |
| Tryptone | 1% w/v | 2 g | |
| Yeast extract | 0.5% w/v | 1 g | |
| NaCl | 1% w/v | 2 g | |
| Agar | 0.7% | 1.4 g |
Autoclave at 121 °C for 00:15:00
15m
Store in oven (> 50 °C )