Jun 07, 2025

Public workspaceTitrimetric Estimation of Acetic Acid Concentration Using Standard Sodium Hydroxide Solution

DOI
https://dx.doi.org/10.17504/protocols.io.yxmvmmrwbv3p/v1
  • Durvesh Smita. Burhade1,
  • Paridhi Jain1,
  • Lakshay Saini1,
  • Yash Modi1
  • 1Parul University
  • Durvesh Smita. Burhade: Project Leader
  • Paridhi Jain: Researcher
  • Lakshay Saini: Researcher
  • Yash Modi: Finance for this Project
  • Durvesh Burhade Lab
Durvesh Burhade
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DOI: https://dx.doi.org/10.17504/protocols.io.yxmvmmrwbv3p/v1
Protocol Citation: Durvesh Smita. Burhade, Paridhi Jain, Lakshay Saini, Yash Modi 2025. Titrimetric Estimation of Acetic Acid Concentration Using Standard Sodium Hydroxide Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.yxmvmmrwbv3p/v1
Manuscript citation:
Burhade, D., Jain, P., Saini, L., Mathur, V., & Modi, Y. (2025). Comprehensive Review of Fundamental Biochemical Techniques: Integration of Quantification, Separation, and Analytical Methods in Biomolecular Studies (1.2). Open Access 2025. https://doi.org/10.5281/zenodo.14872899
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 06, 2025
Last Modified: June 07, 2025
Protocol Integer ID: 219736
Keywords: Titration , Biochemistry , titrimetric estimation of acetic acid concentration, acetic acid concentration, strength of acetic acid, acetic acid, accurate titration method, using standard sodium hydroxide solution, strong base titration, standard sodium hydroxide solution the protocol, phenolphthalein as an acid, weak acid, titrimetric estimation, classical volumetric approach for weak acid, acid, burette preparation, step from burette preparation, ch₃cooh, phenolphthalein, standard sodium
Funders Acknowledgements:
Yash Modi
Grant ID: ORP-BIOCHEMISTRY-2024/25-v2
Abstract
The protocol presents a simple and accurate titration method to determine the concentration and strength of acetic acid (CH₃COOH) using a standard sodium hydroxide (NaOH) solution. The procedure employs phenolphthalein as an acid-base indicator and follows the classical volumetric approach for weak acid–strong base titration. Key reagents and laboratory glassware are detailed, and each step from burette preparation to final endpoint detection is clearly described.
Guidelines
IN THIS EXPERIMENT, WE ADDED

Normality of NaOH: 0.12 Normality
Volume of NaOH: We need to find by titration (Range: 6.0-7.5)
Normality of Acetic acid mix: We need to calculate it
Volume of the acid mixture: 10 mL

The manuscript (CITATION) describes a titration between acetic acid (CH₃COOH) and sodium hydroxide (NaOH) using phenolphthalein as an indicator. However, in the calculation section, it mistakenly references H₂SO₄ (sulfuric acid) and uses its equivalent weight (49 g/equiv), which is inaccurate for acetic acid.
Materials
Sodium hydroxide solution (NaOH) is used in titration to neutralise.
Acetic acid solution (CH3COOH) is an acid in titration.
Phenolphthalein is used as an indicator.


Troubleshooting
Safety warnings
Wear a Lab Coat & Purple Nitrile Gloves while handling solutions
Before start
All the Glassware & Tools should be autoclaved
Titration Through NaOH base & Acetic Acid
Clean the burette thoroughly, wash it with distilled water and finally rinse it with sodium hydroxide solution.
Fill sodium hydroxide solution into the burette through a funnel above the zero mark.
Remove any air gap from the nozzle of the burette by forcing the solution out forcefully from the burette nozzle.
Note the initial reading by keeping the eye at the same level as the solution’s meniscus.
Pipette out Amount10 mL of Acetic Acid solution into a washed and dried conical flask. Always wash the pipette with water and then rinse it.

Add 1-2 drops of phenolphthalein indicator to the conical flask. Titrate the acid with sodium hydroxide solution till a very faint permanent pink colour is obtained. Add sodium hydroxide solution in small amounts initially and then dropwise.
Read the lower meniscus of the solution in the burette again and record it as the final reading.
Repeat the procedure until three concordant readings are obtained.
Lastly Calculate strength of Acetic Acid = equivalent weight x normality
For Calculations use C1 V1 = C2 V2 Formula
OBSERVATION


Amount of solution took


Structure of All the chemical used in the experiments

Protocol references
To Learn more about this method, read this paper

(PDF) Comprehensive Review of Fundamental Biochemical Techniques: Integration of Quantification, Separation, and Analytical Methods in Biomolecular Studies

Cite the Article

Burhade, D., Jain, P., Saini, L., Mathur, V., & Modi, Y. (2025). Comprehensive Review of Fundamental Biochemical Techniques: Integration of Quantification, Separation, and Analytical Methods in Biomolecular Studies (1.2). Open Access 2025. https://doi.org/10.5281/zenodo.14872899
Acknowledgements
I would like to acknowledge my team in the Open Research Project (ORP), who made this protocol Possible