Jun 04, 2026
Tissue Coring for Microarray and Immunohistochemistry Staining
- Fiona Senchyna1
- 1Buck Institute for Research on Aging
External link: https://www.jove.com/t/3620/production-tissue-microarrays-immunohistochemistry-staining
Protocol Citation: Fiona Senchyna 2026. Tissue Coring for Microarray and Immunohistochemistry Staining. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2lymz6plx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 28, 2026
Last Modified: June 04, 2026
Protocol Integer ID: 318147
Keywords: Tissue Microarray, Immunohistochemistry, immunohistochemistry staining coring of tissue, tissue microarray, immunohistochemistry staining coring, high throughput analysis of multiple tissue, microarray, immunohistochemistry staining, digitalization within the human protein atlas, human protein atlas, multiple tissue, coring, high throughput analysis, tissue
Abstract
Coring of tissue for microarray and immunohistochemistry staining allows for high throughput analysis of multiple tissues. The following protocol was part of Production of Tissue Microarrays, Immunohistochemistry Staining and Digitalization Within the Human Protein Atlas
Preparation of Tissue
Select relevant formalin fixed paraffin embedded material (tissue or cell samples) including corresponding hematoxylin stained tissue section.
Mark relevant area on the tissue section. It is recommended to have a freshly cut hematoxylin stained section corresponding to the paraffin block.
Design the template used for TMA production. Randomize the samples within the template to avoid artifacts caused by technical problems such as coverage of the antibody over the whole TMA section and sectioning problems. Add additional orientation markers to the template for orientation.
Organize the tissues according to the template.
Coring of Tissue
To be able to obtain a standardized length of the tissue cores, mark the stylet eg 4.5 mm. Guidelines for spacing between sample centers:
core size 0.6 mm – 0.8-1.0 mm
core size 1.0 mm – 1.8-2.0 mm
core size 1.5 mm – 2.0-3.0 mm
core size 2.0 mm – 2.5-4.0 mm
It is recommended to leave 2.5-3.0 mm margins on each side of the paraffin block to avoid cracking of the paraffin.