Nov 01, 2021
TIMSTOFPro Crosslinking Optimized for PhoX
This protocol is a draft, published without a DOI.
- Johannes Hevler1
- 1Utrecht University
- LCMSMethods.org Version 2
Protocol Citation: Johannes Hevler 2021. TIMSTOFPro Crosslinking Optimized for PhoX. protocols.io https://protocols.io/view/timstofpro-crosslinking-optimized-for-phox-bzncp5aw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: November 01, 2021
Last Modified: November 01, 2021
Protocol Integer ID: 54692
Keywords: timstofpro crosslinking, samples on timstof, timstof pro, proteomic, linking sample, µl sample loop, timstof, lc system
Abstract
crosslinking.m:
Method to run cross-linking samples on TimsTof pro instrument. Method and energies are specifically
optimized for PhoX cross-linker reagent (by Richard Scheltema and Markus Lubeck (Brucker))
Standard_DDA_PASEF_1.1sec_cycletime_2segm_1st_15min_nospectra.m:
Method for classical bottom-up (proteomics) experiments. Optimized for LC systems that are operated
without a trap and are euqipped with a 5 µL sample loop (flowrate 0.400 µL/min), as the Method is segmented: The
first 15 min of the method no spectra are saved. To further reduce the file size the noise filtering for Tims is turned on. (by Richard Scheltema and Markus Lubeck (Brucker))
Troubleshooting
MS_methods_TimsTof (1).zip