Jun 22, 2023
TIMSTOF Compass 4.0 Methods - LCMS and MALDI - OrsburnLab 2023
- Benjamin Orsburn1
- 1The Johns Hopkins University School of Medicine
- LCMSMethods.org Version 2
Protocol Citation: Benjamin Orsburn 2023. TIMSTOF Compass 4.0 Methods - LCMS and MALDI - OrsburnLab 2023. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9p8zzg3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 22, 2023
Last Modified: June 22, 2023
Protocol Integer ID: 83870
Keywords: defaulttimstof40-tmt-highsensitivity-pasefscope diapasef method, maldi instrument methods between different timstof, timstof-40-shortgradient-dia ddapasef method for normal high input, timstof-40-shortgradient-dia ddapasef method, ms method for timstof flex, defaultdda-tims40-high-sensitivity, timstof flex system with easynlc1200, timstof flex system, maldi instrument method, different timstof, orsburnlab 2023 the method, timstof flex, timstof, ms method, 500ng proteomics load, mass spectrometer, evosep one whisper for ultralow input, imaging mass spectrometer
Disclaimer
No guarantees are implicit or implied in the use of these methods. For research use only.
Abstract
The methods included here are in support of research submissions from the www.OrsburnLab.org group in 2023 from a TIMSTOF Flex system with EasyNLC1200, EvoSep One and MALDI (1) front end sources.
Methods include:
pasefRIQ used for SCoPE-MS (DefaultTIMSTOF40_TMT_highsensitivity_pasefSCOPE
diaPASEF methods optimized for the EvoSep One - diaPASEF-shortgradient-1800V
the same - optimized for EasyNLC 1200 using a 10um emitter - TIMSTOF_40_shortgradient_DIA
ddaPASEF method for normal high input (100ng - 500ng proteomics loads) -DefaultDDA_TIMST40_5_6_2023
ddaPASEF methods optimized for the EvoSep One Whisper for ultralow input immunoenriched samples - DefaultDDA_TIMS40_high_sensitivity
A MALDI-MS method for TIMSTOF Flex to test the effectiveness of direct transfer of MALDI instrument methods between different TIMSTOF imaging mass spectrometers:
20220925_SCZ_GelMethod_AngelLab_transfer.m
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