Sep 09, 2020
Thermal cycling of the PCR
- 1ETHZ - ETH Zurich
- XPRIZE Rapid Covid Testing
Protocol Citation: philippe.bechtold 2020. Thermal cycling of the PCR. protocols.io https://dx.doi.org/10.17504/protocols.io.bk6jkzcn
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: September 09, 2020
Last Modified: September 09, 2020
Protocol Integer ID: 41899
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Incubation: 00:10:00 at 55°C
Initial Denaturation 00:03:00 at 92°C
45 cycles of the follwoing:
00:00:25 at 55°C
00:00:02 95°C
Fluorescence is measured at the end of each 55°C period.