Apr 14, 2020
The role of multiprotein bridging factor 1 (MBF1c) in Funariaceae species under salt stress
- Maria Victória Magalhães de Vargas1,2,
- Guilherme Afonso Kessler de Andrade1,2,
- Sara Navarrete Bohi Goulart1,2,
- Bruna Mota Bernardes1,2,
- Filipe Victoria1,2
- 1Núcleo de Estudos da Vegetação Antártica (NEVA), Universidade Federal do Pampa (UNIPAMPA), São Gabriel, RS, Brazil;
- 2neva@unipampa.edu.br
Protocol Citation: Maria Victória Magalhães de Vargas, Guilherme Afonso Kessler de Andrade, Sara Navarrete Bohi Goulart, Bruna Mota Bernardes, Filipe Victoria 2020. The role of multiprotein bridging factor 1 (MBF1c) in Funariaceae species under salt stress. protocols.io https://dx.doi.org/10.17504/protocols.io.be3sjgne
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Other
We attempted this protocol, but could not get it to work in our group
Created: April 14, 2020
Last Modified: April 14, 2020
Protocol Integer ID: 35666
Keywords: Abiotic Stress, expression factors, Funaria hygrometrica, Physcomitrella patens, Physcomitrium acutifolium, qPCR,
Abstract
Bryophytes are one of the most successful plants in nature and can tolerate several environmental stresses, such as saline stress. MBF1 is a transcription factor that may be involved in reprogramming transcription, which in turn may confer tolerance to stress conditions. The objective of this study was to quantify PaMBF1c under saline stress in three species of moss:Funaria hygrometrica,Physcomitrium acutifolium, andPhyscomitrella patens. All moss species underwent 12 h and 24 h treatment with salt at different concentrations, followed by qRT-PCR experiments.
In F. hygrometrica, the MBF1c gene was up-regulated when subjected to 12 h salt treatment at a medium concentration, and may be an important factor for the tolerance of plants to NaCl concentrations. InP. acutifolium,24 h NaCl treatment at of 400, 600, and 800 mM resulted in a gradual up-regulation of MBF1c expression, reflective of the increasing NaCl concentration. InPhyscomitrella,the expression profile of MBF1c showed a down-regulation when treated with 200, 400, and 800 mM NaCl, suggesting that MBF1c may not interfere with the saline stress response in this plant.
The diversity of these expression profiles demonstrate that the MBF1c response is not universal neither among plants nor between species of the same family, as shown in the mosses studied.
Attachments
Materials
MATERIALS
Qubit™ dsDNA HS Assay KitInvitrogen - Thermo FisherCatalog #Q32851
GoTaq(R) Hot Start Polymerase, 2500uPromegaCatalog #M5006
Qubit RNA HS Assay KitThermo Fisher ScientificCatalog #Q32852
PureLink™ RNA Mini KitThermo Fisher ScientificCatalog #12183020
High-Capacity RNA-to-cDNA™ KitThermo FisherCatalog #4387406