Dec 15, 2021

Public workspaceTest protocol II V.7

This protocol is a draft, published without a DOI.
  • 1University of Georgia
Abigail Elizabeth
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Protocol Citation: Abigail Elizabeth 2021. Test protocol II. protocols.io https://protocols.io/view/test-protocol-ii-bzvzp676Version created by Abigail Elizabeth
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this protocol
Created: November 08, 2021
Last Modified: December 15, 2021
Protocol Integer ID: 54937
Abstract
The purpose of this protocol is to demo the NMRFAM template in protocols.io

The scope of this protocol applies to the NAN Knowledgebase groups.


Here's a protocol:
CITATION
Edison AS, Colonna M, Gouveia GJ, Holderman NR, Judge MT, Shen X, Zhang S (2021). NMR: Unique Strengths That Enhance Modern Metabolomics Research.. Analytical chemistry.
LINK
https://doi.org/10.1021/acs.analchem.0c04414

Guidelines
Responsibilities....
Protocol materials
ReagentWater, uHPLC grade
Reagent100% methanol
ReagentMethanol Optima™ LC/MS Grade Fisher ChemicalFisher ScientificCatalog #A456-4
ReagentWater Optima™ LC/MS Grade Fisher Chemical™Fisher ScientificCatalog #W6-4
Safety warnings
Type pertinent safety info. Refer to safety protocols as seen below:
Protocol
The process of introducing safety attitudes questionnaire (SAQ)
NAME
The process of introducing safety attitudes questionnaire (SAQ)
CREATED BY
Ying Li

Before start
Type pertinent info about what the user should know before starting the protocol. Refer to protocols as seen below:
Protocol
C. elegans bleaching solution preparation
NAME
C. elegans bleaching solution preparation
CREATED BY
Cristian Riccio

Use 80:20 MeOH:H2O for this step. This is not easy to access by machine.
If you haven't already, make a solution with the following components:

Concentration80 % volume Reagent100% methanol
Concentration20 % volume ReagentWater, uHPLC grade

Mix
Optional
If you haven't already, make a solution with the following components:

Concentration80 % volume ReagentMethanol Optima™ LC/MS Grade Fisher ChemicalFisher ScientificCatalog #A456-4
Concentration20 % volume ReagentWater Optima™ LC/MS Grade Fisher Chemical™Fisher ScientificCatalog #W6-4

Mix
Optional
Use this piece of equipment:

Equipment
Eppendorf™ 5810R Centrifuge
NAME
Centrifuge
TYPE
Eppendorf
BRAND
02-262-8187
SKU
https://www.fishersci.com/shop/products/eppendorf-5810r-centrifuge-rotor-packages-16/022628187
LINK


Centrifigation






Citations
Edison AS, Colonna M, Gouveia GJ, Holderman NR, Judge MT, Shen X, Zhang S. NMR: Unique Strengths That Enhance Modern Metabolomics Research.
https://doi.org/10.1021/acs.analchem.0c04414