Feb 19, 2026
TBI-ADRD Mouse Husbandry
- Kierra Eldridge1,
- Amandine Wislowsky2,
- Gustavo A. Gomez2,
- Andre Obenaus2,
- Paul R. Territo1
- 1Stark Neuroscience Research Institute, Division of Clinical Pharmacology, School of Medicine, Indiana University;
- 2Division of Biomedical Sciences, School of Medicine, University of California, Riverside
- TBI ADRD UCR/IU
Protocol Citation: Kierra Eldridge, Amandine Wislowsky, Gustavo A. Gomez, Andre Obenaus, Paul R. Territo 2026. TBI-ADRD Mouse Husbandry. protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlk1m61g5r/v1
Manuscript citation:
TBI ADRD Closed Head Injury (CHI) Standard Operating Procedure. dx.doi.org/10.17504/protocols.io.14egnrqw6l5d/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: January 28, 2026
Last Modified: February 19, 2026
Protocol Integer ID: 241673
Keywords: Mouse, Husbandry, Standard Operating Procedures, mild TBI, CHI, tbi adrd closed head injury, husbandry protocol for tbi, adrd mouse husbandry, adrd mouse husbandry this document, adrd mice, tbi, mice, husbandry protocol, standard operating procedure
Funders Acknowledgements:
NIH NINDS
Grant ID: 7R01NS119605-04
NIH NINDS
Grant ID: 5R01NS135556-03
NIH NINDS
Grant ID: 1RF1NS138032-01
Disclaimer
The authors have nothing to disclose
Abstract
This document describes the housing conditions and husbandry protocol for TBI-ADRD mice. These conditions are standardized to promote reproducibility and are associated with conditions used in the following protocol: "TBI ADRD Closed Head Injury (CHI) Standard Operating Procedure". dx.doi.org/10.17504/protocols.io.14egnrqw6l5d/v1
Attachments
appendix 1.pdf
51KB
Guidelines
Photoperiod:
- 12 hours light, 12 hours dark
- Light cycle is either between 6 am and 6 pm or 7 am and 7 pm
Materials
- Irradiated Andersons Enrich-o’Cobs – Product Code 4EB (1/4” cob + paper rolls) (URL: https://assets.andersonsplantnutrient.com/pdf/The-Andersons_Enrich-oCobBrochure_web.pdf)
- Bio-Huts from Bio-Serv #K3352 (URL: https://www.bio-serv.com/product/Bio_Huts.html)
- SAFE Blocks Small (mouse, Aspen wood) Safe-Lab (URL: https://safe-lab.com/safe_en/products/enrichment.php)
- Diet 5K52 - LabDiet® JL Rat and Mouse/Auto 6F (URL: https://www.labdiet.com/product/detail/5k52-labdiet-jl-rat-and-mouse-auto-6f)
- Water: Reverse Osmosis processed, pH 6.8-7
Troubleshooting
Scope
The scope of this SOP includes:
Husbandry protocol (Housing, Social Needs, Enrichment, Diet, Water)
Responsibilities
- SOP Author: responsible for production of the SOP and its described procedures
- SOP Owner: responsible for reviewing and approving changes to the SOP
- Lab staff and students: responsible for following the procedure
- NO CHANGES to this SOP are authorized without the explicit approval from the SOP owner
Housing conditions
Mice are group housed, up to 5 per cage, in ventilated shoebox cages with 75 in2 floor area and 5.5” height. Cage change is weekly where new food, nesting, and enrichment materials are provided.
Temperature:
- 22.2 ± 8°C
Relative humidity:
- 20-80%
Bedding and Nesting:
- Irradiated Andersons Enrich-o’Cobs – Product Code 4EB (1/4” cob + paper rolls) (URL: https://assets.andersonsplantnutrient.com/pdf/The-Andersons_Enrich-oCobBrochure_web.pdf)
Enrichment:
- Bio-Huts from Bio-Serv #K3352 (URL: https://www.bio-serv.com/product/Bio_Huts.html)
- SAFE Blocks Small (mouse, Aspen wood) Safe-Lab (URL: https://safe-lab.com/safe_en/products/enrichment.php)
Photoperiod:
- 12 hours light, 12 hours dark
- Light cycle is either between 6 am and 6 pm or 7 am and 7 pm
Diet/Water
- Diet 5K52 - LabDiet® JL Rat and Mouse/Auto 6F (URL: https://www.labdiet.com/product/detail/5k52-labdiet-jl-rat-and-mouse-auto-6f)
- Water: Reverse Osmosis processed, pH 6.8-7
Appendices
Appendix 1: SOP Record Log