Jul 12, 2020

Public workspaceTAP agar plate preparation

This protocol is a draft, published without a DOI.
  • 1Ronin Institute
Joao Vitor Molino
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Protocol Citation: Joao Vitor Molino 2020. TAP agar plate preparation. protocols.io https://protocols.io/view/tap-agar-plate-preparation-big8kbzw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 12, 2020
Last Modified: July 12, 2020
Protocol Integer ID: 39168
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Always follow aseptic techniques
  1. Autoclavate freshly prepared TAP media with Concentration1.5 Mass / % volume bacteriological agar with a magnetic bar inside
  2. Mix the flask using a magnetic stirrer to evenly dissolve the agar in the solution
  3. For antibiotic containing plates, cool down the flask temperarute until Temperature55 °C - Temperature60 °C or until it is possible to hold it without hurting the skin.
  4. Add antibiotics in the desired amount, and mix using a magnetic stirrer to evenly dissolve the antibiotic
  5. Pour the molten media in sterile petri dishs. (Keep volume constant among plates).
  6. Wait for media to solidify
  7. Stock at Temperature4 °C -Temperature8 °C until use. (Storage for 2 months was used without noticeable difference)